Synthesis of Silica Nanoparticle and Cd-Based Carboxyl Quantum Dots (SiNP@QDs) Conjugates as Biosensing Platform: A Pleriminary Study

Diaz Ayu Widyasari; Fauzan Aulia; Agustina Sus Andreani; Rizna Triana Dewi; Ni Luh Wulan Septiani; Brian Yuliarto; Siti Nurul Aisyiyah Jenie

Diaz Ayu Widyasari Master Program of Nanotechnology, Graduate School, Institut Teknologi Bandung (ITB), Jl. Ganesha 10, Bandung 40312, West Java, Indonesia
Fauzan Aulia Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK, Serpong, South Tangerang 15314, Banten, Indonesia
Agustina Sus Andreani Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK, Serpong, South Tangerang 15314, Banten, Indonesia
Rizna Triana Dewi Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center (CSC), Bogor, West Java 16911, Indonesia
Ni Luh Wulan Septiani Advanced Functional Materials Research Group, Faculty of Industrial Technology, Institut Teknologi Bandung, Bandung 40132, Indonesia
Brian Yuliarto Advanced Functional Materials Research Group, Faculty of Industrial Technology, Institut Teknologi Bandung, Bandung 40132, Indonesia
Siti Nurul Aisyiyah Jenie Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK, Serpong, South Tangerang 15314, Banten, Indonesia

Keywords: Biosensors, E. coli, quantum dots, silica nanoparticles, silanization

Abstract

Silica nanoparticles (SiNP) are one of the most promising materials for biosensors. The introduction of functional groups make silica nanoparticles highly versatile. In this work, the silica nanoparticle was synthesized using sol-gel method from natural based precursor, which is geothermal precipitate. Based on the characterization result of SiNP, the specific surface area is 138 m2/g and nanoparticle size is 43.56 nm. The XRD result shows SiNP in amorphous phase. The surface of SiNP was modified using silanization method with 3-Aminopropyl triethoxysilane (APTES) as an amino silane to immobilize the quantum dot and E coli antibody. The FTIR analysis shows that amine group in SiNP@QDs surface reacted with carboxyl group of antibodies indicated by the presence of a peak at the wavelength of 1620 cm-1. The detection of E. coli bacteria was carried out using fluorescence spectroscopy through the reduced of maximum intensity of SiNP@QDs-Ab before and after E coli addition at wavelength of 595 nm. The fluorescence intensity of SiNP@QDs-Ab is 633.10 a.u and the intensity is reduced to 281.86 a.u. in the presence of E. coli (1 x 107 CFU/mL).

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